Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
ID:
Password:
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

Agents/Distributors
 Regional Subscription Agents/Distributors
 
Current Topics in Analytical Chemistry   Volumes    Volume 1 
Abstract
New approaches to electrochemical biosensing
Werner G. Kuhr, Narasaiah Dontha, Pankaj Singhal
Pages: 1 - 20
Number of pages: 20
Current Topics in Analytical Chemistry
Volume 1 

Copyright © 1998 Research Trends. All rights reserved

 

ABSTRACT

Over the years, electrochemical detection has been proved to be a powerful tool for the sensitive and selective analysis of biological compounds. Various methods including enzymatically derivatized biosensors, and even direct oxidation or reduction at different electrode surfaces have been exploited to this end. Our laboratory has investigated the use of enzymatically modified carbon fiber microelectrodes for the detection of glutamate, an important neurotransmitter in the central nervous system. Various surface derivatization and characterization techniques have been used to derivatize the carbon surface with the enzyme in a controlled manner, in order to maintain high performance levels of the biosensor. Photopatterning of the electrode surface using maskless photolithography has been used to achieve this controlled derivatization of the carbon electrode surface. Fluorescence microscopy and electrogenerated chemiluminescence have been used to characterize the derivatized surface in terms of its physical and chemical properties. In order to exploit the possibility of direct electrochemical detection of biological molecules, without undergoing any derivatization protocols, other electrode surfaces like copper have been used to detect compounds traditionally considered nonelectroactive at carbon surfaces. These include carbohydrates, amino acids and biological macromolecules like oligosaccharides, oligonucleotides and DNA. A new electroanalytical procedure for signal processing in the frequency domain has also been developed which allows us to detect these compounds with much higher sensitivity and selectivity as compared to any existing electrochemical detection approach. These features are not only desirable in any biosensor, but actually necessary in this application for the detection of the generally sample limited case of oligonucleotides and DNA.

Buy this Article


 
search


E-Commerce
Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Login
Search Products
Browse in Alphabetical Order : Journals
Series/Books
Browse by Subject Classification : Journals
Series/Books

Miscellaneous
Ordering Information Ordering Information
Downloadable forms Downloadable Forms