The influence of cold temperature on nutrients fluxes orientation is investigated in Peach-tree single node cuttings. Exogenous sucrose and sorbitol absorption is estimated in the different tissues of the single node cutting, from trees placed in natural conditions and trees placed in a greenhouse heated at 15ºC (cold deprivation). In natural conditions, from October to December, we observe important correlative inhibitions between the bud and its underlying tissues. From January, one month before budburst, correlative inhibitions are removed and the PCMBS-sensitive absorption potentialities are important, particularly in the bud. In cold deprivation, PCMBS-sensitive absorption potentialities are low whatever the month studied and nutrients fluxes were not divert towards a peculiar tissue. The cold temperature seems necessary to initiate the recovery of some metabolic process involving sucrose transporter and seems to induce, at least partially, correlative inhibitions which would be at the outset of the bud’s growth inhibition in November.