Fourier transform infrared spectroscopy (FTIR) has the potential to mark up the chemical changes of the materials, as almost all the materials contain their signatures in infrared region. Spectroscopy combined with spatial resolution enables the possibility of characterizing samples up to microscopic level. The emerging development of instrumentation to provide spatial information for infrared (IR) spectroscopy, termed as IR microscopy, provides an opening for newer applications in terms of image analysis, novel data processing tools, etc. Characterization of biomaterials using IR spectroscopy has a trace back to 1950s. The advent of FTIR with imaging capability made characterization possible in cartilage tissue and other biological systems. Extensive analysis of chemical constituents of cartilage and tendon, collagen orientation and polarization property of cartilage using FTIR imaging (FTIRI) has been actively explored during the last two decades. Also, studies using specialized instrumentations like synchrotron FTIR imaging have been attempted to understand the characteristics of biological samples like cartilage. This review covers most of those investigations on cartilage with FTIRI to characterize the same in terms of component characteristics and quantification, collagen orientation, zonal boundary determination, influence of mechanical compression on tissue nature and its correlation to other techniques in last 20 years.