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Current Topics in Biotechnology   Volumes    Volume 10 
Collagenase production by Aspergillus sclerotiorum URM-5792 using coffee waste as a medium for solid-state fermentation
Kethylen Barbara Barbosa Cardoso, Anna Gabrielly Duarte Neves, Juanize Matias da Silva Batista, Romero Marcos Pedrosa Brandão Costa, Ana Lúcia Figueiredo Porto
Pages: 27 - 31
Number of pages: 5
Current Topics in Biotechnology
Volume 10 

Copyright © 2019 Research Trends. All rights reserved

Collagenases are remarkably important in the biotechnological industry since they have a wide spectrum of applications, and though various natural enzymes purified from animal and plant sources have been studied, nowadays microorganisms have become one of the main sources of enzyme production, particularly filamentous fungi. This study aims at the production of collagenase by Aspergillus sclerotiorum, using the solid-state fermentation technique with coffee waste as a substrate. The results obtained demonstrate significant production of collagenase (679.44 U/ml), which was also found to have an alkaline optimum pH (8) and an optimum temperature at 45 °C. Thus, it is concluded that this microorganism has a reasonable biotechnological value when it comes to protease and collagenase production. These preliminary results are of great value, aiming its application.
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