Spot blotch (SB), caused by the hemi-biotrophs fungal pathogen Cochliobolus sativus, substantially reduces global barley production. To better understand the mechanisms of barley to resist this disease, changes in salicylic acid (SA) and heat shock protein 90 (Hsp90) were monitored during compatible/incompatible barley interaction with C. sativus at different time points of disease development using the quantitative real-time PCR technique (qRT-PCR). The data showed that the SA signaling was activated in both resistant and susceptible genotypes 24 hours post infection (hpi) as compared with non-inoculated plants. However, with or without pathogen pretreatment, SA significantly increased (P = 0.001) in the resistant genotype ‘Banteng’ compared with the susceptible one ‘WI2291’. RT-PCR analysis revealed that Hsp90 expression increases in the resistant and susceptible genotypes over the inoculation time points, with maximum expression (5.9 and 1.89 folds respectively) after 6 dpi. Hsp90 expression was paralleled by an increase in leaf SA content as shown by the test coincidence (F3,32 = 3.86, P = 0.77). Taken together, our data strengthen the idea that SA signaling and Hsp90 play a role during barley-C. sativus interaction.
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