ABSTRACT Nanoparticles (Np) containing trans-[RuCl([15]aneN4)NO]Cl2 complex, a nitric oxide donor agent, were prepared by double-emulsion solvent evaporation method using poly-(D,L lactic-co-glycolic acid) (PLGA). The Np had a mean diameter of 830 ± 18.0 nm smooth surfaces and spherical shape. The process yield and encapsulation efficiency were 62.0 ± 5.0% and 51.0 ± 5.0%, respectively. A simple reversed-phase high-performance liquid chromatographic assay for the trans-[RuCl([15]aneN4)NO]Cl2 complex was developed to determine the encapsulation efficiency. The nitrosyl ruthenium complex was analyzed using a 5 µm Shimadzu RP 18 column (250 x 4 mm). The mobile phase was trifluoroacetic acid (HTFA) (0.5%) pH 2.5: methanol (85:15 v/v) at a flow rate of 1.0 mL.min-1. Detection was carried out at 255 nm at room temperature (25 oC). Linearity was obtained in the range from 2.5 to 250 µg.mL-1. The within and between-day assays coefficient of variation and relative error was less than 10% The results indicated that the method presents good precision and accuracy. The present method was validated and shown to be useful for the analysis of trans-[RuCl([15]aneN4)NO]Cl2 encapsulated in PLGA nanoparticles.
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