We assessed the effect of live and inactivated influenza vaccines and live polio vaccine admixed with adjuvant (chitosan glutamate) for cytoplasmic expression of several pattern recognition receptors (PRRs) in human dendritic cells. The receptors studied included endosomal TLRs (TLR3, TLR7, TLR8, TLR9), NLRs (NOD1, NOD2) and RLRs (Rig-1, Rig-2). Our data suggest that one of the possible mechanisms of immune response polarization to Th-1 type after immunization with live and inactivated viral vaccines in combination with chitosan glutamate as adjuvant is increased activation of pattern recognition receptors localized in dendritic cells.