Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
ID:
Password:
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

Agents/Distributors
 Regional Subscription Agents/Distributors
 
Trends in Cancer Research   Volumes    Volume 11 
Abstract
HER2-mediated branching morphogenesis is dependent on AKT activation
Kathleen M. Woods Ignatoski, Radha Arghal, Natalie M. Wolters Niemi, Geeta Mehta, Stephen P. Ethier
Pages: 71 - 85
Number of pages: 15
Trends in Cancer Research
Volume 11 

Copyright © 2016 Research Trends. All rights reserved

ABSTRACT
 
HER2 (or erbB2) is a receptor tyrosine kinase in the epidermal growth factor receptor (EGFR) family that is overexpressed in approximately 30% of human breast cancers (BrCa) where overexpression correlates with a poor prognosis. However, the consequences of HER2 expression in normal mammary epithelia, where it is expressed physiologically at low levels, are not understood. We investigated whether the HER2-initiated pathways found in BrCa cells played a role in the development of the mammary gland. We studied the ability of a primary human mammary epithelial (HME) cell to undergo morphological changes and form polarized branching structures in Matrigel. We show that there was an increase in both total and tyrosine phosphorylated HER2 levels in normal HME cells that exhibited changes in colony morphology. A HER2-overexpressing cell line in Matrigel also produced differential colony morphogenesis compared to vector control. The morphology change included multi-cellular branches that had a ductal appearance in hematoxylin and eosin (H&E)-stained thick sections and in electron micrographs and secreted a milk-like substance. Using inhibitors and constitutively activated mutants, we determined that phosphoinositide 3-kinase (PI3K) is necessary but not sufficient, and that AKT is both necessary and sufficient to produce the morphological changes associated with overexpression of HER2. Our data suggest that HER2 signaling, via AKT, plays a role in normal mammary gland development.
Buy this Article


 
search


E-Commerce
Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Login
Search Products
Browse in Alphabetical Order : Journals
Series/Books
Browse by Subject Classification : Journals
Series/Books

Miscellaneous
Ordering Information Ordering Information
Downloadable forms Downloadable Forms