Thermal polycondensation with formation of metal halide salts as a driving force has proved to be an interesting method to synthesize alternating poly(ester amide)s constituted by glycolic acid and ω-amino acid units. These new materials may be used for biomedical applications and consequently biocompatibility tests are essential to follow further developments. Cytotoxicity, cell adhesion and cell proliferation studies on a representative poly(ester amide) derived from 6-aminohexanoic acid have been carried out using either L929 (fibroblast type) and Hep-2 (epithelial type) cell lines. Furthermore, cell viability tests have been performed with liquid extracts of the polymer. Results are promising for future biological applications of the studied material.