Seven lignan compounds (1-7) were isolated from the leaves of Phyllanthus myrtifolius Moon. and were evaluated for their in vitro antifungal activities against an opportunistic filamentous fungus, Fusarium oxysporum, using the Clinical and Laboratory Standard Institute (CLSI) (M38-A2) standard method. The lignans phyllamyricin C (1), retrojusticidin B (2), phyllamyricin A (3), phyllamyricin F (4), justicidin B (5), phyllamyricin E (6) and piscatorin (7) were found to be active against F. oxysporum. Their minimum inhibitory concentration (MIC) values were in the range of 4.0-32.0 μg mL-1 and minimum fungicidal concentration (MFC) values were in the range of 8.0-62.5 μg mL-1. The isolated lignans also have inhibitory activity on the conidial germination of F. oxysporum. The results strongly suggest that lignans isolated from the leaves of P. myrtifolius can be developed as natural antifungal agents.
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