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Current Topics in Biotechnology   Volumes    Volume 13 
Pichia pastoris whole cells in the biotransformation of monoterpenes
Bheatriz Santos Ferrage, Wagner Lopes, Graziela Jardim Pacheco, Danielle Frias Ribeiro Bisaggio, Aline de Souza Ramos
Pages: 95 - 104
Number of pages: 10
Current Topics in Biotechnology
Volume 13 

Copyright © 2022 Research Trends. All rights reserved

Biotransformation is a biotechnological process that allows obtaining other substances from a precursor using biocatalysts, which can be microorganisms. In this work, the yeast Pichia pastoris was used to catalyze reactions with three substrates, the monoterpenes α-pinene, β-pinene, and γ-terpinene. The most promising substrate was selected for studies for a shorter reaction period and using immobilized cells in calcium alginate. Initially, the reactions were carried out for 48 h using free cells in an aqueous medium containing glucose and substrate. After extraction with dichloromethane, substrates and products were analyzed by gas chromatography coupled to a mass spectrometer. Under these conditions, the three substrates underwent near total conversion. Biotransformation with α-pinene as substrate proved to be more promising because only one product was formed, 2-phenylethanol, which has a rose aroma and is of great industrial interest. The monoterpene α-pinene was tested in reactions for 24 h, but the conversion to 2-phenylethanol was around 50%. Then, the cells were immobilized in calcium alginate and used in 24-hour reactions. However, other products were formed, different from 2-phenylethanol. Therefore, it is concluded that obtaining 2-phenylethanol through the biotransformation of the monoterpene α-pinene by free cells of P. pastoris can be a promising bioprocess.
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