Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

 Regional Subscription Agents/Distributors
Trends in Developmental Biology   Volumes    Volume 13 
Transcription factors show a prolonged dwell time in the non-dividing Xenopus oocytes
K. Javed, Tooba Gull, J. B. Gurdon
Pages: 77 - 86
Number of pages: 10
Trends in Developmental Biology
Volume 13 

Copyright © 2020 Research Trends. All rights reserved

Transcription factors have a major function in orchestration of gene expression programs which lead to the formation of new cell types and tissues in a developing organism. Their mode of action is not known; in particular it is not known whether they continuously bind to and dissociate from their target DNA or chromatin or whether, once bound they remain that way for hours or days. Clarification of this uncertainty connects closely with the stability of gene expression, a characteristic of the differentiated state of the cells, which for most cell types changes very little during life. The dwell time of transcription factors on DNA or chromatin which has been largely analysed by fluorescent recovery after photobleaching (FRAP) experiments do not measure the stability of gene expression. We have recently introduced a way of determining the duration of dwell time as well as the stability of the binding, in contrast to what was concluded by FRAP experiments. We found that in Xenopus oocytes, the gene expression can be stable for hours to days. We also have found that the formed complexes resist the competition by another template. It is suggested here that the binding of transcription factors may be stable and can promote cell differentiation at least for the non-dividing cell. In this article we aim to uncover the potential mechanism which may contribute to such stability of gene expression in nondividing cells.
Buy this Article


Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Search Products
Browse in Alphabetical Order : Journals
Browse by Subject Classification : Journals

Ordering Information Ordering Information
Downloadable forms Downloadable Forms