Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
ID:
Password:
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

Agents/Distributors
 Regional Subscription Agents/Distributors
 
Current Topics in Peptide & Protein Research   Volumes    Volume 14 
Abstract
Expression, purification and crystallization of pore mutants of Ammonium transport protein 1 from Archaeoglobus fulgidus
Daniel Cebo
Pages: 13 - 17
Number of pages: 5
Current Topics in Peptide & Protein Research
Volume 14 

Copyright © 2013 Research Trends. All rights reserved

ABSTRACT
 
Ammonium transport proteins are highly conserved families of integral membrane proteins present in all domains of life. Functional studies with members of this family have yielded controversial results with respect to the chemical identity of the transported species (NH4+ or NH3) as well as to the classification of the proteins as channels or secondary active transporters. In this work different point mutations of Ammonium transport protein 1 gene (Amt-1) have been produced by site-directed mutagenesis. Then heterologous overexpression in Escherichia coli has been used to produce proteins and they have been purified to electrophoretic homogeneity. Crystals of Amt-1 have been obtained by sitting-drop vapor diffusion method.
Buy this Article


 
search


E-Commerce
Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Login
Search Products
Browse in Alphabetical Order : Journals
Series/Books
Browse by Subject Classification : Journals
Series/Books

Miscellaneous
Ordering Information Ordering Information
Downloadable forms Downloadable Forms