Chitosan is a linear polysaccharide derived by deacetylation of very abundant chitin. Chitosan is metabolized into non-toxic D-glucosamines by lysozymes present in the body and hence is biodegradable. Hence, chitosan has attracted significant interest in tissue regeneration applications. Various processes have been developed to form porous structures, needed for guiding cell growth. The processes include freeze-drying, thermo-gelation and electrospinning. Recent advances show that the porous structures formed from these processes are useful in cell colonization, however, show different cellular responses and mechanical characteristics. Chitosan has also been blended with various macromolecules to alter the biological and mechanical characteristics. This article summarizes the recent advances in processing chitosan-based scaffolds and their biomechanical alterations.