We report the persistence and the clearance of two high-risk human papillomaviruses (HPVs) HPV-16 and HPV-35 in a patient. The progress of HPV-16 infection was confirmed by an increased expression of viral E7 gene and cellular CDKN2A and CDKN1A genes. Simultaneously, HPV-35 clearance in this patient is shown. The expression of these viral and cellular genes could be helpful predictors of cervical lesions.