Atrazine is one of the most widely used herbicides to control broadleaf and grassy weeds in many crops around the world. However, its indiscriminate use will lead to the accumulation of atrazine in soil and drinking water, resulting in severe impacts on human health. Hence, it is crucial to develop a better remediation strategy to remove atrazine from the environment. In this research, mycoremediation using fungi, which is both an environmental friendly and a cost-effective way was evaluated to remediate the atrazine. The objective was to determine which fungi species have the potential to tolerate the toxicity of atrazine at various increasing concentration. Eleven fungi species were screened using 10 mg/L of atrazine on potato dextrose agar (PDA). Out of the 11 species, five were found to have greater biomass and were further tested on potato dextrose brooth (PDB) using increasing concentration of atrazine (up to 20 mg/L). Results showed that both Trichoderma erinaceum and Aspergillus nidulans recorded the highest mean dry biomass when exposed to the highest atrazine concentration tested at 20 mg/L, which is 0.117 g and 0.073 g, respectively. This shows both T. erinaceum and A. nidulans can tolerate atrazine up to 20 mg/L, thus having the highest potential to remediate atrazine.