Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

 Regional Subscription Agents/Distributors
Trends in Comparative Biochemistry & Physiology   Volumes    Volume 16 
Tissue-specific acclimation of enzyme activity in the Eastern red spotted newt (Notophthalmus viridescens viridescens)
Nancy J. Berner, John R. Bullock, W. Gray Jerome, Trevor H. Marquand, Christopher L. Waldrup
Pages: 61 - 70
Number of pages: 10
Trends in Comparative Biochemistry & Physiology
Volume 16 

Copyright © 2012 Research Trends. All rights reserved

The Eastern red spotted newt is an ectotherm that is active in winter and acclimation or acclimatization from summer to winter conditions results in behavioral, biochemical and metabolic modifications. Cytochrome c oxidase (CCO) is embedded in the inner mitochondrial membrane and in skeletal muscle, its activity increases in winter. The activity of citrate synthase (CS), a mitochondrial matrix enzyme, also increases in winter. The present study had two main purposes: 1) to include liver in our analysis; and 2) to determine the mechanisms by which the changes in enzyme activity are accomplished. To address goal 1 we measured the activity of CCO, CS and lactate dehydrogenase in liver tissue from summer- and winter-acclimatized newts. To address goal 2, in liver and skeletal muscle of summer- and winter-acclimatized newts we a) determined mitochondrial volume density and cristae surface area via TEM; b) measured the expression of genes encoding CCO and CS via rt-PCR; c) determined membrane fatty acid composition via gas chromatography of fatty acid methyl esters. We found: 1) CCO activity was higher in summer than winter in liver; 2) mitochondrial volume density decreased in muscle in winter while cristae surface area increased in winter in liver; 3) CCO activity was correlated with changes in membrane composition, not gross mitochondrial characteristics or gene expression; 4) CCO activity in liver did not correlate well with the parameters we measured; 5) direct quantification of mitochondrial properties by TEM is important.
View Full Article  


Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Search Products
Browse in Alphabetical Order : Journals
Browse by Subject Classification : Journals

Ordering Information Ordering Information
Downloadable forms Downloadable Forms