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Trends in Organic Chemistry   Volumes    Volume 17 
Modified ECHO probes for highly specific RNA detection in living cells
Akimitsu Okamoto
Pages: 45 - 57
Number of pages: 13
Trends in Organic Chemistry
Volume 17 

Copyright © 2014 Research Trends. All rights reserved

The regulation of the mechanisms by which genomic information dictates cellular behavior is one of the most important and urgent research topics in the postgenomic era. Chemically modified nucleic acids would be key players in sensitive, quantitative, and real-time detection of specific transcripts. In this article, we review recent advances in nucleic acid detection using exciton-controlled hybridization-sensitive fluorescent oligonucleotide (ECHO) probe technologies. In ECHO probes, a hybridization-dependent fluorescent nucleotide regulated by the H-aggregation of thiazole orange dyes is incorporated into specific sequence contexts. ECHO probe serves as a fluorescent detection readout for target nucleic acids. In addition, auxiliary chemical functional modules such as pH sensitivity, photolabile ability, and enhanced hybridization property have been introduced into ECHO probes to accommodate a broad range of biological applications.
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