Otitis externa (OE) is an infectious disease frequently detected in dogs, caused by coagulase-positive Staphylococcus species such as S. aureus and S. pseudintermedius. As the effectiveness of the currently available antimicrobial drugs is compromised by the molecular mechanisms of bacterial resistance, new options are necessary to provide proper treatment. Here we provide evidence of the antimicrobial potential of Curcuma longa ethanolic extract against S. aureus strains isolated from dogs with otitis. C. longa is widely appreciated for culinary purposes, especially in Asia, and is widely investigated for its antitumoral and anti-inflammatory properties. We conducted classical phytochemical analyses and minimal inhibitory concentration experiments. Given the scarcity of data concerning bacterial species of veterinary source and interest, our data becomes even more relevant.