Filamentous fungi naturally produce a broad range of plant cell wall degrading enzymes that are widely used in several industrial sectors such as pulp and paper, biofuels or textiles. In this study, the enzymatic profiles of twelve fungal species after being grown on three plant polysaccharides were compared with one another. Our results demonstrate significant differences in the set of enzymes the filamentous fungi produce during growth on plant polymers and provide leads for improved biotechnology applications.