Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

 Regional Subscription Agents/Distributors
Current Topics in Phytochemistry   Volumes    Volume 18 
Bruceine B-induced apoptosis of lung cancer cells NCI-H292 through endoplasmic reticulum stress, death receptor and mitochondria-dependent signaling pathways
Wen-Zhe Li, Feng Yu, Chuan Wang, Mei Dong, Zhi-Yu Ni, Mao-Chang Ji, Hiromasa Kiyota
Pages: 11 - 18
Number of pages: 8
Current Topics in Phytochemistry
Volume 18 

Copyright © 2022 Research Trends. All rights reserved

Bruceine B is a kind of quassinoid isolated from the fruits of Brucea javanica (L.) Merr. (Simaroubaceae). The anti-proliferative effects of bruceine B on NCI-H292 cells were estimated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Furthermore, the bruceine B-induced apoptosis in NCI-H292 cells was determined by fluorescence microscope through TUNEL staining with DAPI and by flow cytometry analysis via staining with Annexin V-FITC/PI. The expression levels of proteins related to apoptosis pathway were measured by western blot analysis. Our results revealed that bruceine B showed significant anti-tumor activity on lung cancer in vitro via up-regulation of p53, Bax, caspase-8 and caspase-3. Meanwhile, we also used GRP78 and caspase-12 which indicated that bruceine B induced cell death through endoplasmic reticulum (ER) stress. These results demonstrated that bruceine B induced cell apoptosis through ER stress, death receptor and mitochondria-dependent signaling pathways.
Buy this Article


Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Search Products
Browse in Alphabetical Order : Journals
Browse by Subject Classification : Journals

Ordering Information Ordering Information
Downloadable forms Downloadable Forms