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Current Topics in Toxicology   Volumes    Volume 18 
Isolation and identification of cadmium-resistant bacteria that degrade crude oil
Efendy Chew, Wong Rui Rui, Ong Ghim Hock, Wong Kok Kee
Pages: 135 - 139
Number of pages: 5
Current Topics in Toxicology
Volume 18 

Copyright © 2022 Research Trends. All rights reserved

Three bacterial isolates were successfully screened and shown to have the ability to biodegrade crude oil and are resistant to cadmium at 0.1 mg/L. However, when the Cd concentration was increased from 0.1 mg/L to 1.0 mg/L, only one isolate, isolate C, was able grow. Gram staining results showed the isolate C to be Gram-negative. The DNA extracted from isolate C was amplified using a pair of 16s rDNA primers. The amplified DNA sequences were used to construct a phylogenetic tree. Analysis of the phylogenetic tree showed that 16s rRNA sequences from isolate C have the highest similarity to the 16s rRNA sequence of Pseudomonas aeruginosa.
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