Gastric cancer is one of the most common and deadly cancers worldwide, with a highly varied incidence and mortality by regions. The majority (over 60%) of gastric cancers is associated with infectious agents, including the bacterium Helicobacter pylori and the Epstein-Barr virus (EBV). Several meta-analyses have shown that human papillomavirus (HPV) infection is a high-risk factor for carcinogenesis at some anatomic sites, including the skin, genital tract, respiratory tract, and disparate anatomic sites of the digestive tract. Some studies have investigated HPV infection in gastrointestinal cancer. The objective of our work is to determine the prevalence of HPV and EBV infections in gastric cancer in an Algerian population. Seventy-one paraffin blocks of gastric cancer tissues from patients in western Algeria were evaluated for the presence of EBV and HPV genomes by polymerase chain reaction. EBV and HPV was absent in all specimens (0%). This study shows that human papillomavirus and Epstein Barr virus infections are not risk factors to develop gastric cancer in Algeria.