EPR imaging grew up to be a matured tool to investigate spatial distributions and EPR spectra simultaneously. The most remarkable advances in hardware are the use of low microwave frequency and the introduction of loop gap cavity to in vivo EPR imaging. On the other hand, the spectral-spatial EPR imaging has been developed for studying on radicals with multiline and/or multi-component EPR spectra. This means the wide feasibility of EPR imaging for almost all paramagnetic species occurring naturally in physical chemistry.