The synthesis of 1,2-O-isopropylidene acyl glycerol from 1,2-O-isopropylidene glycerol catalysed by Amberlite XAD-7 adsorbed lipase from Pseudomonas sp. AK was studied. The immobilized lipase preparation showed a maximum conversion degree of 75% of 1,2-isopropylidene lauroyl glycerol. The synthetic activity of the immobilized lipase was optimum at the temperature range of 30-50^oC in n-hexane with an agitation rate of 150 rpm. The   optimum ratio of 1,2-O-isopropylidene glycerol to lauric acid was  2:1 (mol/mol). Fatty acids of carbon numbers C12 – C18  which are readily miscible in apolar organic solvents show higher conversion degree. Apolar solvents with the Log P values of 3.0 and  above, also resulted higher conversion degree. Salt hydrates with higher a_w does not promote the catalytic activity of the immobilized lipase. The addition of CaCl₂.2H₂O or CaCl₂.0/2H₂O helped to control the water content in the system. On the other hand, a high a_w saturated salt solution of 0.963 was needed to pre-equilibrate the lipase preparation for optimum conversion degree. Under the optimized reaction conditions, a maximum conversion degree of almost 90% was obtained after 24 h. The repeated use of the immobilized preparation for synthesis of 1,2-O-isopropylidene lauroyl esters was more than 60 repeated usage or 2 months maintaining the conversion degree in the range of 85 –90%.