The tumor microenvironment in which ovarian malignant neoplasia develops has been described as enriched with a broad spectrum of proinflammatory cytokines and chemokines that may influence the clinical state and prognosis. The aim of the study was to correlate the dosage of interleukins IL-2, IL-5, IL-6, IL-8, IL-10, and nitric oxide (NO) metabolites in serum, peritoneal and intracystic fluid with hematological parameters of patients with malignant ovarian neoplasia. We evaluated 29 patients diagnosed with primary ovarian malignancy. IL-2, IL-5, IL-6, IL-8 and IL-10 concentrations were quantified by enzyme-linked immunosorbent assay (ELISA). Colorimetric assay was performed for the measurement of nitric oxide (NO) metabolites. Cytokine concentrations were evaluated in serum, peritoneal and intracystic fluids prior to surgical treatment. The results were analyzed by Spearman test. Evaluation of serum showed an inverse correlation of IL-8 and IL-10 with absolute value of lymphocytes, and direct correlation with NLR and PLR; there was also an inverse correlation of iNOS with NLR and direct relationship with red blood cell count (RBC). Evaluation of intracystic fluid showed an inverse correlation of IL-6 and RBC, leukocytes and lymphocytes; direct correlation of IL-8 with platelet-lymphocyte (PLR); inverse correlation of NO metabolites with neutrophil-lymphocyte (NLR) and PLR, and direct correlation with lymphocytes. Evaluation of peritoneal fluid showed an inverse correlation of IL-6 and RBC, absolute lymphocyte value, and hemoglobin. Some cytokines, such as IL-6, IL-8, IL-10 and nitric oxide metabolites, correlate with blood count parameters that influence disease prognosis, such as anemia, absolute lymphocyte value, NLR, and PLR. Thus, these cytokines could be considered new prognostic factors in ovarian cancer, guiding the clinical oncologist for better treatment and follow-up, and being potential targets in the study of new treatments.
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