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Current Topics in Pharmacology   Volumes    Volume 21 
In-vitro evaluation of thymol derived from Trachyspermum ammi against acetaminophen-induced hepatotoxicity and its therapeutic applications for lymphatic filariasis
Abel Arul Nathan, Anand Setty Balakrishnan
Pages: 35 - 47
Number of pages: 13
Current Topics in Pharmacology
Volume 21 

Copyright © 2017 Research Trends. All rights reserved

Individuals with lymphatic filariasis (LF) show elevated levels of pro-inflammatory cytokines and altered expressions of inflammatory genes, and are prone to secondary bacterial infection. Also markers of liver dysfunction were reported to be high with increased filarial infection. Acetaminophen (Paracetamol), an anti-pyretic drug was prescribed to LF individuals along with diethylcarbamazine (DEC) and albendazole (ALB) to alleviate pain and filarial fever within the therapeutic dosage. It is unclear, whether parasite-secreted toxins augment the action of acetaminophen and enhance liver dysfunction. Hence, an agent which can attenuate the acetaminophen toxicity during filarial infection is warranted. Trachyspermum ammi (T. ammi) is the richest source of thymol, reported to have the anti-filarial lead molecule. In the present study we evaluated the effects of thymol against acetaminophen-induced hepatotoxicity in in-vitro settings. In addition, we examined the synergistic effects of thymol against bacterial infection, free radicals and cytokine production. Our results reveal that acetaminophen (250 µg/ml) induces significant reduction in the viability of WRL-68 liver cells compared to cells without treatment. However, thymol at the same concentration restores the cell viability significantly (p = 0.031) by attenuating the toxicity within 24 h. Thymol inhibits the expression of Interleukin-6 (p = 0.043) and Interleukin-8 (p = 0.048) in WRL-68 cells and thereby provides maximum protection to liver cells from inflammatory insults. Calorimetric analysis shows the ability of thymol in scavenging hydrazyl (p = 0.004) and hydrogen peroxide(p = 0.008) free radicals efficiently. Thus thymol derived from T. ammi may be a good therapeutic agent in reducing the toxicity of acetaminophen, and may also aid in LF treatment and management in addition to the existing drugs.
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