An electrochemical sensor based on poly (threonine)-modified carbon nanotube paste electrode (PTMCNTPE) was developed for the simultaneous resolution of dopamine (DA) and uric acid (UA). Electropolymerization method was used to prepare poly (threonine), which was characterized by field emission scanning electron microscopy (FESEM) in order to understand the surface morphology. The electrochemical detection of DA and UA was carried out by cyclic voltammetry (CV) and differential pulse voltammetry (DPV).  The developed sensor provides sharp and clear peaks for the electrooxidation of DA and UA as compared to bare carbon nanotube paste electrode (BCNTPE). The linear plot of DA was obtained  in the range from 1 × 10^-5 to 5 × 10^-5 M and 6 × 10^-5 to 1.9 × 10^-4 M  with a detection limit of 7.7 × 10^-7 M and limit of quantification of 25 × 10^-7 M. The practical application of the developed sensor was verified as exact and sensitive for the resolution of DA in pharmaceutical samples.