Muscarinic acetylcholine receptors (mAChRs) are G-protein-coupled receptors mainly activated via the parasympathetic pathway. These receptors are widely known to mediate functions within both central and peripheral nervous system, activated by the neurotransmitter acetylcholine (ACh). Well known studies suggested the contribution of ACh in regulating cardiovascular functions via various pathways; however, the role of mAChRs was limited to the AChM2R, AChM3R, and AChM4R subtypes. In contrast, AChM1R subtype has not been sufficiently studied in the cardiovascular system due to its abundance in the central nervous system and its implication in many physiological and pathological brain functions. Recently, studies revealed the presence of AChM1R in the cardiovascular system directly using knockout models, or indirectly treating with AChM1R selective agonist/antagonist. This mini-review aims to summarize recent findings in the role of AChM1R in cardiovascular function and diseases.