The tooth-coating solution has been developed as a cosmetic. The tooth-coating solution is composed of rosin, copal and shellac which are suggested to have anti inflammatory efficacies. The pharmacological efficacies of the tooth-coating solution and its components on the proliferation of T cell mitogen-stimulated peripheral blood mono-nuclear cells (PBMCs) derived from healthy subjects were evaluated. The effects of the components on production of seven kinds of Th1/Th2/Th17 cytokines from the stimulated PBMCs were also measured. The tooth-coating solution and its ingredients attenuated the proliferation of T cell mitogen-stimulated PBMCs. Rosin, copal and shellac significantly decreased the concentration of IL-6 in the culture supernatant of the stimulated PBMCs (p < 0.05). The tooth-coating solution, copal and shellac also decreased the concentration of TNF-α in the supernatant of the stimulated PBMCs (p < 0.05). From these observations, we suggest that the tooth-coating solution has potency to prevent inflammation by attenuation of T cell activation and subsequent production of inflammatory cytokines, which may be beneficial for suppression of periodontal disease and stomatitis.