Psychotria viridis Ruiz & Pav. (P. viridis) is a ritual plant used as one of the ingredients in the preparation of the ayahuasca drink, which is consumed by indigenous and religious groups during sacred rituals in countries of South America. Studies have shown that the extract of this plant has therapeutic effects on diseases such as depression, anxiety disorders, and chemical dependencies. The aim of this study is to evaluate the effects of P. viridis leaf extracts on nociception. Male Wistar rats (180-200 g) were used in this study. To induce the experimental inflammatory pain, carrageenan (200 µg/paw) was injected into the paw. Nociception was measured using the mechanical paw pressure test. P. viridis extracts used include methanolic (PVM), ethyl acetate (PVA) and hexanic (PVH). A surprising variation in the antinociceptive effect of the three extracts evaluated (PVM, PVA e PVH) was observed, a fact that may occur due to the large number of substances contained in them. Based on the dose-response curve, the peak of action and the doses with the greatest antinociceptive effect of the extracts were determined, that is 9.6 mg (time; t = 2.5 h) for PVM; 2 mg (t = 4 h) for PVA; and 4.8 and 19.2 mg (t = 0.5 h and 2.5 h, respectively) for PVH (there were two points with significant differences). In the subsequent stage, the peak action of the extracts in the doses with the greatest antinociceptive effect coincided with the peak action of carrageenan. PVH and PVM were able to partially reverse the nociceptive effect of carrageenan, while PVA showed a promising total reversal of the effect of carrageenan. It is possible to conclude that P. viridis has a high analgesic potential and therefore, is promising in the development of new drugs for pain control.