Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
ID:
Password:
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

Agents/Distributors
 Regional Subscription Agents/Distributors
 
Current Topics in Peptide & Protein Research   Volumes    Volume 3 
Abstract
Modulation of protease activity by NO-mediated S-nitrosylation
Paolo Ascenzi, Marco Colasanti, Tiziana Persichini, Fabio Polticelli, Giorgio Venturini, Fabrizio Bortolotti, Enea Menegatti
Pages: 181 - 188
Number of pages: 8
Current Topics in Peptide & Protein Research
Volume 3 

Copyright © 1999 Research Trends. All rights reserved

ABSTRACT

Nitric oxide (NO) is generated in different cell types by the concomitant conversion of L- arginine into L-citrulline through the enzyme NO synthase. NO has been claimed to exert its action in an increasing number of physiological and pathological events. Among others, cellular communication, blood pressure regulation, homeostasis and memory formation. A huge amount of NO is also produced under pathological conditions, such as inflammation and immunological processes. This wide variety of effects is achieved through interactions of NO with some targets via a rich redox and additive chemistry. In particular, it has been shown that NO-mediated S-nitrosylation inhibits the activity of several enzymes, containing Cys residue(s) at their catalytic site, e.g. papain, caspases and cathepsin-B. Moreover, NO may modulate the activity of enzymes containing Cys residues at their regulatory regions. In this respect, NO-mediated S-nitrosylation of the regulatory Cys residues inactivates the viral-encoded aspartyl protease, a crucial enzyme for HIV-1 replication. Finally, the NO-mediated S-nitrosylation of Cys83, the single free sulfhydryl residue present in the fibronectin type-1 and epidermal growth factor-like pair of modules of the tissue-type plasminogen activator (t- PA) does not affect the catalytic (i.e. fibrinolytic) activity, but endows the serine protease with new potent vasodilatory and antiplatelet properties. In this respect, t-PA acts as a macromolecular NO-transporter. Here, the NO-mediated S-nitrosylation of some representative proteases is reviewed.

Buy this Article


 
search


E-Commerce
Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Login
Search Products
Browse in Alphabetical Order : Journals
Series/Books
Browse by Subject Classification : Journals
Series/Books

Miscellaneous
Ordering Information Ordering Information
Downloadable forms Downloadable Forms