Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

 Regional Subscription Agents/Distributors
Current Topics in Biochemical Research   Volumes    Volume 3 
Identification and characterization of PKC- θ interacting proteins
Yael Babichev, Nahum Meller, Sigal Gelkop, Vered Yiflah-Vishkerman, Stephan Witte, Amnon Altman, Noah Isakov
Pages: 243 - 250
Number of pages: 8
Current Topics in Biochemical Research
Volume 3 

Copyright © 2000 Research Trends. All rights reserved


Members of the protein kinase C (PKC) family of serine/threonine protein kinases have been implicated in numerous cellular responses in a large variety of cell types. The  isoenzyme exhibits a relatively restricted pattern of expression with high protein levels found predominantly in hematopoietic cells and skeletal muscle. In addition, it is expressed in T, but not B lymphocytes, and is translocated from the cytosol to the membrane in a cell activation-dependent manner. In the present work we further analyzed whether subcellular localization and/or biological functions of  involve physical interaction with other effector molecules.  was found to interact with the 14-3-3τ protein, a known modulator of signaling effector molecules. Overexpression of 14-3-3τ resulted in the suppression of PKCθ-induced interleukin 2 promoter activation and inhibition of phorbol ester-induced cytosol to membrane translocation of PKCθ. In contrast, 14-3-3τ did not alter PKCθ enzymatic activity in vitro and did not undergo phosphorylation by PKCθ. In a different line of studies we have employed the yeast two hybrid system and identified a cDNA encoding a novel 335-amino acid PKCθ-interacting protein with a calculated mass of 37.5 kDa. This protein, termed PICOT, is expressed in various tissues, including in T cells, and displays an N-terminal thioredoxin-homology domain which is required for the interaction with PKC. Current studies are aimed at the characterization of the mode of interaction of PKCθ and either 14-3-3τ or PICOT, and the effects of the interaction on the subcellular localization and biological functions of theses three proteins.

Buy this Article


Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Search Products
Browse in Alphabetical Order : Journals
Browse by Subject Classification : Journals

Ordering Information Ordering Information
Downloadable forms Downloadable Forms