ABSTRACT The bolus intravenous injection of a novel medium-chain triglyceride: fish oil emulsion (MCT: FO) was recently proposed as a tool for the rapid repletion of cell phospholipids in long-chain polyunsaturated ω3 fatty acids. In the present report, the time course and efficiency for the generation of unesterified fatty acids from this emulsion, as catalysed in vitro by lipoprotein lipase, are investigated. The results obtained with the MCT: FO emulsions are compared to those recorded, under identical experimental conditions, with either a pure fish oil emulsion (FO) or a 5:4:1 (w/w) medium-chain triglyceride: long-chain triglyceride: fish oil emulsion (MLF). The results document the following hierarchy in the generation of unesterified fatty acids from the MCT: FO emulsion C8:0 > C10:0 > C16:0 > C22:6ω3 > C20:5ω3. The fractional release of C20:5ω3, C22:5ω3 and C22:6ω3 was higher in the case of diglycerides than triglycerides. Differences were also found in the efficiency for the generation of the same unesterified fatty acid relative to its initial content in each emulsion. These findings document that the MCT: FO emulsion indeed represents a suitable tool (i) for the rapid and large lipoprotein lipase-catalyzed generation of unesterified C8:0 and C10:0, susceptible to act as nutrients and, hence, spare the catabolism of long-chain polyunsaturated ω3 fatty acids, (ii) to allow the incorporation in cells of lipid particles still containing the latter fatty acids and (iii) to generate, nevertheless, unesterified ω3 fatty acids that could also participate in the enrichment of cell phospholipids in these molecules.
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