Gene trapping in mouse embryonic stem (ES) cells is an efficient method for identifying new genes and examining their functions for vertebrate, and helping people to understand the similar genes involved in human. We have developed two trap vectors and a culture system to differentiation the embryonic stem cell and selection them in vitro. By using those gene trap vectors and the selection embryonic stem cells in vitro, we identified several novel genes involved in mouse development. We have reported the genes PRDC which is expresses in commissural neurons in the developing spinal cord, midnolin which is expressed in the mesencephalon, IZP6 which is expressed in neural cells, and will report the gene helg which is strong expressed in the neural cells during embryogenesis, effected the development of the neural system and the somites during mouse development. Analyzing these genes obtained by gene trapping, will help us to elucidate the functions of the genes for vertebrate in development.
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