ABSTRACT A limited number of microorganisms attack and degrade the highly branched polymer found in wood, lignin. Many organisms that cause extensive degradation of lignin belong to the group called the white-rot fungi. This group of fungi have been used in the process of bioremediation. Interest on fungal bioremediation has predominantly focused on the lignin degrading or ligninolytic enzymes of phanerochaete chrysoporium, lignin peroxidase and manganese peroxidase. These enzymes are associated with degradation of organic pollutants and decolourisation of recalcitrant organic dyes. A third ligninolytic enzyme, laccase, has received increasing attention. White-rot fungi that produce laccase can also degrade organic pollutants and decolourise certain dyes on both a laboratory scale and in bioreactor studies. A purified preparation of lacasse decolourises the disazo dye Chicago Sky Blue and the vinly sulphonyl dye, Remazol Briliant Blue R.
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