The purpose of this study was to clarify the relationship between the development of the cerebral masticatory center, ingestive behavior, and tooth eruption during the weaning period of puppies. The results showed that electrical stimulation of the cerebral cortex induced no tongue or jaw movements in puppies tested during the first 15 days after birth, but there were sucking-like movements from 19 to 23 days and chewing-like movements after the eruption of primary teeth.