Shoot and adventitious root cultures of Saxifraga stolonifera were established and the production of bergenin in the tissues was determined. Although the concentrations of bergenin in the shoot cultures were low, the bergenin concentration in the adventitious root cultures reached 0.69 % dry weight, which was higher than that of field grown plants. Furthermore, in vitro grown S. stolonifera roots were found to produce two volatile compounds that were identified as 2-phenylethyl alcohol and eugenol by solid phase microextraction headspace analysis. These fragrance molecules were not detected in the naturally cultivated plants.
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