Radiata pine represents one of the primary plantation species, because produce timber much faster than natural forests and its resource has traditionally been used to provide wood chips for the pulp and paper industry and as a raw material for the packaging industry. Within the past 30 years, however, both New Zealand and Chile began employing a silvicultural regime that emphasized the selective thining of plantations and the pruning of trees beginning  early in the growth cycle. This regime has resulted in fast growing trees while facilitating the production of large volumes of clear wood which compares favorably to many pinus species . Tremendous genetic gain has already been secured in radiate pine tree improvement programs using conventional selection and  breeding methods. However, a number of highly desirable characteristics that are not readily available in the breeding population may be introduced using genes from other organisms. Various techniques have been designed for the transfer of foreign genetic material into plant cells. Actually, efforts are being made to accelerate the genetic  improvement of forest trees through the development of molecular genetic techniques and the application of bioinformatic methods. Micropropogation of Pinus radiata is an attractive alternative for the amplification and multiplication of selected genotypes. Recent advances in plant production by in vitro tissue culture and the application of reinvigoration treatment have indicated that industrial plant production is feasible starting of select genotypes. The study of genomic DNA methylation-demethylation during aging and reinvigoration using radiata pine as model allow concluding that observed changes in the extent of methylation during the ageing ( increasing of degree of DNA methylation) and reinvigoration processes (decreasing in the extent of DNA methylation) indicate that reinvigoration could be a consequences of opposite epigenetic modifications to that during ageing. On the other hand, DNA methylation can act directly as a epigenetic mechanism and it can explain phenotypic variations after tissue culture without genetic damage.