The amiloride-sensitive and -insensitive components of salt- and acid-induced responses in mouse non-dissociated taste cells were examined using the whole-cell clamp technique and localized taste stimulation method. Both amiloride-sensitive and -insensitive components were involved in salt-induced depolarizing responses of two mouse strains (C57BL/6 and BALB/c). The amiloride-insensitive salt response consisted of multiple components, including Na⁺ influx and inhibition of K⁺ channels. All acid-induced responses in taste cells examined were insensitive to amiloride, but were markedly suppressed by the Cl^- channel blocker, NPPB. These results suggest that multiple, different transduction mechanisms are involved in and contribute to salty and sour transductions.