In the present study, IgG2b-type anti-HBsAg antibodies (2G3, 6E7) complexed to commercially available Hepatitis B virus (HBV) vaccine (GenHevac B, Pasteur, France) at varying concentration of antibodies (0,5; 1; 1,5; 5; 10; 20 µg respectively) were found to considerably stimulate primary antibody response in mice. However, no such stimulation was observed with IgG1 antibody (2C8). The intensity of immune response was implicated to increase with the increasing dose of antibody in vaccine-antibody complex comparing with those immunized by vaccine alone. Administration of HBV vaccine with IgG2b antibody influenced the subclass distribution and specificity of antibody response. These results indicate that antigen specific monoclonal antibodies may have an important role to induce better immune response to HBV vaccine and this effect may depend on the affinity or isotype of monoclonal antibodies.