Growth inhibition of AmpC ß-lactamase producing E. coli and metallo ß-lactamase producing P. aeruginosa using acidic and neutral oil components of Aloe vera was investigated. Clinical isolates of E. coli and P. aeruginosa were used. Dried leaves of Aloe vera were extracted using soxhlet method and chemically separated into acidic and neutral oil components. Growth inhibition was determined by the turbidometric method. There was a progressive antimicrobial effect against the isolates with an increase in the concentration of the extracts. The acidic component had more effect on the test organisms and E. coli showed higher percentage of inhibition at all concentrations of the extracts. The result showed that the components of Aloe vera can be used to fight against antibiotic resistant organisms.