The study investigated the diesel-oil degrading potentials of single and mixed bacterial cultures from diesel polluted soil. The isolates were purified and identified as members of the genera Pseudomonas, Bacillus, Staphylococcus and Proteus using their cultural and biochemical characteristics. They were screened for diesel oil utilization using the vapour phase transfer method and the plates with very high turbidity in modified mineral salts medium containing diesel as the sole source of carbon and energy were selected as pure cultures, and their growth profiles monitored. This was done by monitoring the pH, optical density and total viable count for 14 days at 2 days interval. The effect of diesel oil and glucose on the isolates was monitored. Adherence and emulsification tests were as well performed on the isolates. The results showed changes in pH, optical density and total viable count after the 14 days incubation period. The isolates used adherence and emulsification as mechanisms for oil uptake with the highest percentage obtained in the consortium for both tests. Diesel oil and glucose had little or no effect on the lag phase of the isolates but the exponential phases were stimulated at all concentrations except for Bacillus sp. whose exponential phase was stimulated at 0.5% and depressed at 1.0% and 2.0% in diesel oil and depressed at 0.8% and 1.6% in glucose.