The bioconversion of acetophenone derivatives 1-6 was investigated using whole cells of marine fungi Botryosphaeria sp. Br-09, Eutypella sp. Br-023, Hydropisphaera sp. Br-27 and Xylaria sp. Br-61 isolated from the red alga Bostrychia radicans and Arthopyrenia sp. SGPY-41, Penicillium sp. SMA2-8, Pestalotiopsis sp. SMA2-C isolated from brown alga Sargassum sp. Asymmetric reduction produced the enantiopure (R)- or (S)-alcohols 7-12 with high enantiomeric excess (>99 % ee). This study describes the first investigation with marine-derived fungi recovered from algae for biocatalytic reduction. The fungus Botryosphaeria sp. Br-09 showed excellent reductions for ortho-acetophenone derivatives 1-6.