A study was performed in the Tissue Culture Laboratory, Eastern University, Sri Lanka for understanding the histological response of in vitro cultured rhizome buds of ginger (Zingiber officinales Rosc.) for shoot organogenesis. Young rhizome buds of field grown local variety of ginger were collected, surface sterilized and then rhizome buds of 1 cm length were cultured on MS medium containing 3.0 mg/L 6-benzylaminopurine (BAP) and 0.5 mg/L 1-naphthaleneacetic acid (NAA). The results revealed that shoot buds initiated from the cultured rhizome explants, and nine days were taken for the initiation of the buds. Histological study clearly showed that longitudinal sections of the rhizome bud cultured on MS medium had inner and outer zones separated by a layer, and that the microshoots originated from the meristematic tissues of the cultured rhizomes of ginger.
Buy this Article