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Current Topics in Phytochemistry   Volumes    Volume 9 
Micropropagation of Anoectochilus formosanus Hayata in vitro and pharmacological and chemical investigations
Xiao-Ming Du, Nobuto Irino, Takuhiro Uto, Osamu Morinaga, Yukihiro Shoyama
Pages: 79 - 87
Number of pages: 9
Current Topics in Phytochemistry
Volume 9 

Copyright © 2008 Research Trends. All rights reserved

Several Anoectochilus species have been used in Chinese folk medicines. A. formosanus Hayata is grown in Taiwan and Okinawa and has been used for hypertension, lung and liver diseases, and under developed children as a folk remedy. However, since the shortage of these resources occurred recently, we micropropagated A. formosanus by tissue culture technique to produce plantlets.  The extract of A. formosanus showed significant activity in decreasing the levels of the cytosolic enzymes LDH, GOT and GPT, and the result evidently suggested that A. formosanus possessed prominent hepatoprotective activity against CCl4-induced hepatotoxicity. Moreover, in the results of the test using aurothioglucose-induced obese mice, the extract showed a significant antihyperliposis effect. In order to confirm the active component, the extract of A. formosanus cultured in vitro was purified to isolate kinsenoside (1) guiding with hepatoprotective activity against CCl4 toxicity in primary cultured rat hepatocytes as a major and active compound. Hepatoprotective and antihyperliposis activities of kinsenoside were assayed resulted that kinsenoside is the real pharmacologically active component.
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