Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

 Regional Subscription Agents/Distributors
Current Topics in Analytical Chemistry   Volumes    Volume 9 
Analysis of protein damage based on the fluorometry of tryptophan residue
Kazutaka Hirakawa, Shiori Inoue
Pages: 57 - 62
Number of pages: 6
Current Topics in Analytical Chemistry
Volume 9 

Copyright © 2012 Research Trends. All rights reserved

Photosensitized protein damage is an important process in phototoxicity and the mechanism of photodynamic therapy. Fluorometry could be used for the evaluation of the protein photo-damaging activity of a photosensitizer. In this study, we designed a procedure for the analysis of protein damage by fluorometry using a water-soluble protein, human serum albumin (HSA). HSA has one tryptophan residue, which is a strongly fluorescent amino acid and is easily oxidized by photochemical reaction. The oxidation of HSA results in a decrease of the fluorescence intensity of the tryptophan residue. These properties are appropriate to probe oxidative protein damage. On the basis of these properties of HSA and tryptophan, an analytical procedure for protein damage is proposed.
View Full Article  


Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Search Products
Browse in Alphabetical Order : Journals
Browse by Subject Classification : Journals

Ordering Information Ordering Information
Downloadable forms Downloadable Forms