Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
ID:
Password:
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

Agents/Distributors
 Regional Subscription Agents/Distributors
 
Current Topics in Steroid Research   Volumes    Volume 9 
Abstract
Ecdysone 20-monooxygenase activity during embryogenesis of the tobacco hornworm, Manduca sexta
Christopher A. Drummond, Stan L. Smith
Pages: 67 - 72
Number of pages: 6
Current Topics in Steroid Research
Volume 9 

Copyright © 2012 Research Trends. All rights reserved

ABSTRACT
 
The conversion of ecdysone (E) to 20-hydroxyecdysone (20E), the active arthropod molting hormone, via the cytochrome P450-dependent enzyme, ecdysone 20-monooxygenase (E20M), initiates the metabolic and physiological events that occur during molting and metamorphosis throughout insect postembryonic development. The present study used an in vitro radioassay to examine E20M enzyme activity throughout the 120-hour period of embryogenesis in the tobacco hornworm, Manduca sexta. E20M enzyme activity increases significantly at two points during embryogenesis, at 12 hours and 72 hours into embryogenesis. At 72 hours, E20M activity achieves its maximal level of activity observed during embryogenesis, subsequently decreasing to basal levels for the rest of the period. Interestingly, the 72-hour peak in enzyme activity corresponds to the time at which the accumulation of the 20-hydroxylated ecdysteroid observed in embryogenesis is at its peak, and this is the period between the secretion of the first and second embryonic cuticle.
View Full Article  


 
search


E-Commerce
Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Login
Search Products
Browse in Alphabetical Order : Journals
Series/Books
Browse by Subject Classification : Journals
Series/Books

Miscellaneous
Ordering Information Ordering Information
Downloadable forms Downloadable Forms