Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

 Regional Subscription Agents/Distributors
Current Topics in Pharmacology   Volumes    Volume 9  Issue 1
Development of a simple screening system for phytoecdysteroids in the environmental pollutants using mammalian cell line
Masaharu Komatsu, Seiichi Ando, Shota Takumi, Tatsuhiko Furukawa, Yumiko Komatsu, Kohji Aoyama, Toru Takeuchi
Pages: 81 - 89
Number of pages: 9
Current Topics in Pharmacology
Volume 9  Issue 1

Copyright © 2005 Research Trends. All rights reserved


Chemical pollution including endocrine disruptors is serious environmental problem for human and wildlife. Although screening systems of estrogenic endocrine disruptors have been established, available system of ecdysone-disrupting compounds for arthropod has not been established yet.   Here we developed a novel screening system of the endocrine disruptors for arthropod using mammalian cells. A human embryonic kidney cell line (HEK293 cells) stably expressing both Drosophia ecdysone receptor (EcR) and mammalian retinoid X receptor (RXR) was transfected with a plasmid possessing ecdysone response elements and reporter gene lacZ to generate EcR-293/lacZ cells. The EcR-293/lacZ cells were used for screening of ecdysone-disrupting compounds. Brief principle of this screening system is as follows: ecdysone-disrupting compound is initially bound to EcR/RXR heterodimer, these complexes of ligand and receptor are subsequently bound to ecdysone response elements and induce the transcription of lacZ gene, and β-galactosidase of EcR-293/lacZ cells is finally produced. Using this reporter gene assay system, we demonstrated that a phytoecdysone polypodine B induced β-galactosidase activity as well as a phytoecdysone ponasterone A and a synthetic ecdysteroid muristerone A. However, ecdysone, 2-deoxy-20-hydroxyecdysone, and 20-hydroxyecdysone, which are biologically active and endogenous ecdysteroid hormones for insects and crustacean, could not induce the β-galactosidase activity. In addition, ponasterone A inducing β-galactosidase activity was partially inhibited by 20-hydroxyecdysone. Furthermore, all-trans-retinoic acid, which is a ligand for RXR, could not affect the reporter gene assay. These results suggest that our developed EcR293/lacZ cells are suitable for screening of environmental phytoecdysteroids which possess ability to be bound to their own EcR/RXR receptor complex.

Buy this Article


Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Search Products
Browse in Alphabetical Order : Journals
Browse by Subject Classification : Journals

Ordering Information Ordering Information
Downloadable forms Downloadable Forms