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Current Topics in Biochemical Research   Volumes    Volume 9  Issue 1
Abstract
cDNA cloning and stage-specific expression of ubiquitin in Physarum polycephalum
Yoshiko Minami, Naruki Hontao, Hiroshi Matsubara
Pages: 99 - 103
Number of pages: 5
Current Topics in Biochemical Research
Volume 9  Issue 1

Copyright © 2007 Research Trends. All rights reserved

ABSTRACT
 
A cDNA library from Physarum plasmodia was screened using an anti-ubiquitin antibody, yielding four different cDNAs (ub1, ub2, ub3, and ub4). Ub 1 cDNA, which consists of 490 nucleotides, encodes a fusion protein of monoubiquitin and C-terminal extension protein. Ub2 has three repeats, while ub3 and 4 have four ubiquitin repeats in the sequences. A high homology was found among the nucleotide sequences in these ubiquitin repeats. These nucleotide sequences encode the same amino acid sequence except for the first ubiquitin sequence in ub3, in which glutamine at position 63 is replaced with histidine. We also examined the expression of each ubiquitin mRNA in different cell stages by real time RT-PCR. Amoebae expressed a high level of ub1 and ub4 mRNA, and spores also included a large amount of ub2 and ub4 mRNA. However, the expression levels of every ubiquitin mRNA in other cell stages were low.
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